My research
Experiment
I am aiming to construct a battery using an artificial photosynthetic reaction system in which coenzyme is incorporated into a circulation regeneration system by a solar cell. In recent years, global warming has become a problem due to the rise in concentrations of greenhouse gases such as CO2 and methane in the atmosphere. Among these greenhouse gases, CO2 is considered to contribute most to the temperature rise of the earth, especially because of its high emissions. At present, as for CO2 fixation method, chemical absorption method, membrane separation method, etc. are being studied. Among these methods, there are various problems such as the cost of heat to be consumed and the durability of the film material is low. On the other hand, in the CO2 immobilization system using enzymes derived from microorganisms, it is unnecessary to consider the cost of light energy and compared with conventional methods such as being able to specifically fix CO2 under mild conditions .It is considered useful. In this study, I try to immobilize CO2 by combining enzymatic reaction by enzymes derived from microorganisms and solar cells that convert solar energy into electric energy.
At first, it was confirmed by HPLC whether CO2 was fixed by enzymatic reaction. I confirmed whether it was transformed into malic acid by enzymatic reaction because it was found that CO2 was fixed when it was reduced from pyruvic acid to malic acid. As a result, Production of malic acid of the same concentration could be confirmed according to the concentration of coenzyme added. It also shows that CO2 at the same concentration as the added coenzyme was immobilized. I could confirm that CO2 immobilization by enzyme reaction.
Then, I will evaluate the oxidation-reduction potential by a three-electrode method using a solar cell. Although I have not done this experiment yet, I will evaluate how much substrate (pyruvic acid) has been reduced to malic acid by confirming the redox peak obtained by the three electrode method. By evaluating this, I can confirm whether the same amount of CO 2 as malic acid was immobilized. Adjusting the voltage is a problem because the voltage accumulated in the solar cell is used. Therefore, I’m planning to adjust the voltage using a function generator.
I am aiming to construct a battery using an artificial photosynthetic reaction system in which coenzyme is incorporated into a circulation regeneration system by a solar cell. In recent years, global warming has become a problem due to the rise in concentrations of greenhouse gases such as CO2 and methane in the atmosphere. Among these greenhouse gases, CO2 is considered to contribute most to the temperature rise of the earth, especially because of its high emissions. At present, as for CO2 fixation method, chemical absorption method, membrane separation method, etc. are being studied. Among these methods, there are various problems such as the cost of heat to be consumed and the durability of the film material is low. On the other hand, in the CO2 immobilization system using enzymes derived from microorganisms, it is unnecessary to consider the cost of light energy and compared with conventional methods such as being able to specifically fix CO2 under mild conditions .It is considered useful. In this study, I try to immobilize CO2 by combining enzymatic reaction by enzymes derived from microorganisms and solar cells that convert solar energy into electric energy.
At first, it was confirmed by HPLC whether CO2 was fixed by enzymatic reaction. I confirmed whether it was transformed into malic acid by enzymatic reaction because it was found that CO2 was fixed when it was reduced from pyruvic acid to malic acid. As a result, Production of malic acid of the same concentration could be confirmed according to the concentration of coenzyme added. It also shows that CO2 at the same concentration as the added coenzyme was immobilized. I could confirm that CO2 immobilization by enzyme reaction.
Then, I will evaluate the oxidation-reduction potential by a three-electrode method using a solar cell. Although I have not done this experiment yet, I will evaluate how much substrate (pyruvic acid) has been reduced to malic acid by confirming the redox peak obtained by the three electrode method. By evaluating this, I can confirm whether the same amount of CO 2 as malic acid was immobilized. Adjusting the voltage is a problem because the voltage accumulated in the solar cell is used. Therefore, I’m planning to adjust the voltage using a function generator.